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KMID : 1094020150320060536
Journal of Veterinary Clinics
2015 Volume.32 No. 6 p.536 ~ p.539
Recovery of In Vivo Matured Oocytes from a Bitch with Hydrometra
Kim Min-Jung

Jo Young-Kwang
Oh Hyun-Ju
Kim Geon-A
Erif Maha Nugraha Setyawan
Choi Yoo-Bin
Lee Seok-Hee
Lee Byeong-Chun
Kim Hyun-Il
Kang Sang-Chul
Abstract
One year old mixed-breed bitch was examined to retrieve in vivo matured oocytes. Laparotomy wasperformed 72 hr after ovulation determined by serum progesterone concentration, and abnormally enlarged left uterushorn was found. Both ovaries had eight corpus lutea, and a total 16 in vivo matured oocytes having perivitelline spacewithin 25 ¥ìm, polar body, and metaphase II nucleus were recovered by flushing oviducts. This is the first study toconfirm in vivo maturation of oocytes from a bitch with hydrometra, which suggests that oocytes recovered from canidswith reproductive disease could be valuable sources for assisted reproductive technologies.
KEYWORD
dog, hydrometra, oocyte maturation, oocyte recovery
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